Search results for " Gram-negative bacteria"

showing 8 items of 8 documents

Artemisia arborescens essential oil composition, enantiomeric distribution and antimicrobial activity from different wild populations from the Medite…

2016

International audience; Aerial parts of Artemisiaarborescens were collected from different sites of the Mediterranean area (southwestern Algeria and southern Italy) and the chemical composition of their essential oil (EO) extracted by hydrodistillation was studied by both gas chromatography (GC) equipped with an enantioselective capillary column and GC/mass spectrometry (GC/MS). The EOs obtained were tested against several Listeriamonocytogenes strains. Using GC and GC/MS, 41 compounds were identified, accounting for 96.0-98.8% of the total EO. All EOs showed a similar terpene profile, which was rich in chamazulene, -thujone, and camphor. However, the concentration of such compounds varied …

0106 biological sciencesListeriaBioengineeringMicrobial Sensitivity TestsSettore MED/42 - Igiene Generale E Applicata01 natural sciencesBiochemistry[ CHIM ] Chemical Scienceslaw.inventionTerpeneCamphorchemistry.chemical_compoundlawBotanyOils Volatile[CHIM]Chemical SciencesFood scienceMolecular BiologyEssential oilVolatile compositionbiologyChemotypeMediterranean RegionChemistryChamazuleneBiological activityStereoisomerismGeneral ChemistryGeneral Medicinebiology.organism_classificationArtemisia arborescensEnantiomeric distributionListeria monocytogenesAnti-Bacterial Agents0104 chemical sciences010404 medicinal & biomolecular chemistrySettore MED/18 - Chirurgia GeneraleArtemisiaItalyAlgeriaGram-negative bacteriaMolecular MedicineArtemisiaGas chromatographyEnantiomeric distribution Biological activity Gram-negative bacteria Volatile composition Listeria monocytogenes.010606 plant biology & botanySettore AGR/16 - Microbiologia Agraria
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Antibiotic treatment of infections caused by carbapenem-resistant Gram-negative bacilli: an international ESCMID cross-sectional survey among infecti…

2018

ESGAP, ESGBIS, ESGIE and the CRGNB treatment survey study group.

0301 basic medicineAcinetobacter baumanniiCarbapenemAntibioticsDrug ResistanceDrug resistanceTigecyclineAcinetobacter baumannii; Carbapenem; Carbapenem-resistant Gram-negative bacilli; Combination therapy; Enterobacteriaceae; Polymyxin; Pseudomonas aeruginosa; SurveyPolymyxin0302 clinical medicineSurveys and Questionnairespolycyclic compounds030212 general & internal medicineAcinetobacter baumannii; Carbapenem; Carbapenem-resistant Gram-negative bacilli; Combination therapy; Enterobacteriaceae; Polymyxin; Pseudomonas aeruginosa; Survey; Anti-Bacterial Agents; Carbapenems; Cross Infection; Cross-Sectional Studies; Drug Resistance Bacterial; Gram-Negative Bacteria; Gram-Negative Bacterial Infections; Hospitals; Humans; Microbial Sensitivity Tests; Surveys and QuestionnairesSurveyCarbapenemAcinetobacter baumannii; Carbapenem; Carbapenem-resistant Gram-negative bacilli; Combination therapy; Enterobacteriaceae; Polymyxin; Pseudomonas aeruginosa; Survey; Anti-Bacterial Agents; Carbapenems; Cross Infection; Cross-Sectional Studies; Drug Resistance Bacterial; Gram-Negative Bacteria; Gram-Negative Bacterial Infections; Hospitals; Humans; Microbial Sensitivity Tests; Surveys and Questionnaires; Microbiology (medical); Infectious DiseasesCross InfectionbiologyMicrobial Sensitivity TestCarbapenem-resistant Gram-negative bacilliBacterialantibiotic management carbapenem-resistant Gram-negative bacteriaGeneral MedicineHospitals3. Good healthAcinetobacter baumanniiAnti-Bacterial AgentsInfectious DiseasesPseudomonas aeruginosamedicine.drugHumanMicrobiology (medical)medicine.medical_specialtymedicine.drug_class030106 microbiologyMicrobial Sensitivity TestsFosfomycincarbapenem-resistant Gram-negative bacteria03 medical and health sciencesHospitalEnterobacteriaceaeInternal medicineAnti-Bacterial AgentDrug Resistance BacterialGram-Negative BacteriamedicineGram-Negative Bacterial InfectionHumansCombination therapyCross-Sectional Studiebusiness.industrybiochemical phenomena metabolism and nutritionbiology.organism_classificationbacterial infections and mycosesCross-Sectional StudiesCarbapenemsInfectious disease (medical specialty)Carbapenem-resistant gram-negative bacilli[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologieantibiotic managementbusinessGram-Negative Bacterial InfectionsRifampicin
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Efficacy of a coordinated strategy for containment of multidrug-resistant Gram-negative bacteria carriage in a Neonatal Intensive Care Unit in the co…

2021

AbstractBackgroundAntimicrobial resistance in neonatal intensive care unit (NICU) patients is a threat, due to the frequent use of antimicrobial treatment and invasive devices in fragile babies. Since 2014 an active surveillance program of multidrug-resistant Gram-negative bacteria (MDR-GNB) carriage has been in place in the five NICUs of Palermo, Italy. In 2017 an increase in the prevalence of MDR-GNB, and in particular of extended-spectrum β-lactamases-producingKlebsiella pneumoniae(ESBL-KP), was observed in “Civico” hospital NICU.AimTo assess the impact of a coordinated intervention strategy in achieving long-lasting reduction of MDR-GNB prevalence in the NICU.MethodsRectal swabs were ob…

0301 basic medicineMicrobiology (medical)Malemedicine.medical_specialtyNeonatal intensive care unit030106 microbiologyExtended spectrum β lactamases producing klebsiella pneumoniaeContext (language use)Drug resistanceMicrobial Sensitivity TestsAntimicrobial resistancelcsh:Infectious and parasitic diseases03 medical and health sciences0302 clinical medicineAntibiotic resistanceMedical microbiologyNeonatal intensive care unitInternal medicineDrug Resistance Multiple BacterialIntensive Care Units NeonatalGram-Negative BacteriamedicineHumansPharmacology (medical)lcsh:RC109-216030212 general & internal medicineNeonatal intensive care unit.Cross InfectionInfection ControlActive surveillance programbusiness.industryResearchPublic Health Environmental and Occupational HealthInfant NewbornAntimicrobialMulti-drug resistant gram-negative bacteriaElectrophoresis Gel Pulsed-FieldInfectious DiseasesCarriageItalyIntervention strategyCarrier StateFemaleSample collectionbusinessGram-Negative Bacterial Infections
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What is the risk of acquiring bacteria from prior intensive care unit bed occupants?

2017

0301 basic medicineRiskmedicine.medical_specialtyLetterMulti drug resistant bacteriaSepsi030106 microbiologyIntensive Care UnitBacterial contamination; Infection; Multi-drug resistant bacteria; SepsisBacterial contamination; Infection; Multi-drug resistant bacteria; Sepsis; Critical Care and Intensive Care MedicineMulti-drug resistant bacteriaCritical Care and Intensive Care Medicinelaw.inventionSepsis03 medical and health sciences0302 clinical medicinelawPatients' RoomSepsisPatients' RoomsAnti-Bacterial AgentHumansMedicineMultidrug-resistant gram-negative bacteriaMED/41 - ANESTESIOLOGIAIntensive care medicineCross InfectionInfection Controlbiologybusiness.industry030208 emergency & critical care medicinemedicine.diseasebiology.organism_classificationIntensive care unitAnti-Bacterial AgentsIntensive Care UnitsEquipment ContaminationbusinessInfectionBacteriaBacterial contaminationHuman
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Fluorescent probes to evaluate the physiological state and activity of microbial biocatalysts: A guide for prokaryotic and eukaryotic investigation

2008

International audience; Many fluorescent techniques are employed to evaluate the viability and activity of microbial cells used in biotechnology. These techniques are sometimes complex and the interpretation of results opened to misunderstanding. Moreover, new developments are constantly proposed especially concerning a more accurate evaluation of the state of the cells including eukaryotic microorganisms. This paper aims at presenting to biotechnologists unfamiliar with fluorescence the principles of these methods and the related possible pitfalls. It focuses on probes of the physical (integrity and fluidity) and energetical (intracellular pH and membrane potential) state of the cell membr…

Cell Membrane PermeabilityMembrane FluidityMESH : Microscopy FluorescenceMESH : Cell MembraneIntracellular pHMESH : Membrane FluidityBiologyApplied Microbiology and BiotechnologyMembrane PotentialsCell membraneIndustrial MicrobiologyMESH : Hydrogen-Ion ConcentrationYeastsGram-Negative BacteriamedicineMESH : Membrane PotentialsMESH : Fluorescent DyesFluorescent DyesMESH : YeastsMESH : Spectrometry FluorescenceCell Membrane[ SDV.BIO ] Life Sciences [q-bio]/BiotechnologyGeneral MedicineHydrogen-Ion ConcentrationMESH : Gram-Negative BacteriaMESH : Industrial MicrobiologyFluorescenceYeastSpectrometry Fluorescencemedicine.anatomical_structureMicroscopy FluorescenceBiochemistryMESH : Cell Membrane PermeabilityNucleic acidMolecular MedicineBiotechnology Journal
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2,6-Disubstituted imidazo[2,1-b][1,3,4]thiadiazole derivatives as potent staphylococcal biofilm inhibitors.

2019

Abstract A class of 36 new 2-(6-phenylimidazo[2,-1-b][1,3,4]thiadiazol-2-yl)-1H-indoles was efficiently synthesized and evaluated for their anti-biofilm properties against the Gram-positive bacterial reference strains Staphylococcus aureus ATCC 25923, S. aureus ATCC 6538 and Staphylococcus epidermidis ATCC 12228, and the Gram-negative strains Pseudomonas aeruginosa ATCC 15442 and Escherichia coli ATCC 25922. Many of these new compounds, were able to inhibit biofilm formation of the tested staphylococcal strains showing BIC50 lower than 10 μg/ml. In particular, derivatives 9c and 9h showed remarkable anti-biofilm activity against S. aureus ATCC 25923 with BIC50 values of 0.5 and 0.8 μg/ml, r…

Indoles3Anti-virulence agentStaphylococcus1-b][1Bacterial growthAnti-Biofilm agentsmedicine.disease_causeSettore BIO/19 - Microbiologia GeneraleGram-Positive Bacteriaimidazo[201 natural sciencesVirulence factorMicrobiology03 medical and health sciencesStaphylococcus epidermidisDrug DiscoveryGram-Negative BacteriaThiadiazolesmedicineStaphylococcal biofilm inhibitorsEscherichia coli030304 developmental biologyPharmacology0303 health sciences4]thiadiazole derivativesbiologyStaphylococcal biofilm inhibitorVirulenceAnti-Biofilm agents; Anti-virulence agents; imidazo[21-b][134]thiadiazole derivatives; Staphylococcal biofilm inhibitors; Anti-Bacterial Agents; Biofilms; Gram-Negative Bacteria; Gram-Positive Bacteria; Indoles; Staphylococcus; Thiadiazoles; Virulence010405 organic chemistryPseudomonas aeruginosaChemistryimidazo[21-b][134]thiadiazole derivativesOrganic ChemistryBiofilmGeneral Medicinebiology.organism_classificationSettore CHIM/08 - Chimica FarmaceuticaAnti-Biofilm agent0104 chemical sciencesAnti-Bacterial AgentsAnti-virulence agentsStaphylococcus aureusBiofilms1 3 4 thiadiazole derivativesEuropean journal of medicinal chemistry
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Inhibitory Activity and Chemical Characterization ofDaucus carotasubsp.maximusEssential Oils

2017

The essential oils (EOs) of green seeds from Daucus carota subsp. maximus growing wild in Pantelleria Island (Sicily, Italy) were characterized. EOs were extracted by steam distillation, examined for their inhibitory properties against food-borne Gram-positive and Gram-negative bacteria and analyzed for the chemical composition by gas chromatography (GC) and mass spectrometry (MS). Undiluted EOs showed a large inhibition spectrum against Gram-positive strains and also vs. Acinetobacter spp. and Stenotrophomonas maltophilia. The minimum inhibition concentration (MIC) was in the range 1.25 – 2.50 μl/ml for the most sensitive strains. The chemical analysis indicated that D. carota subsp…

Sesquiterpene0301 basic medicineSettore AGR/05 - Assestamento Forestale E SelvicolturaMonoterpeneChemical compositionMonoterpeneSettore MED/42 - Igiene Generale E Applicata01 natural sciencesBiochemistryEssential oilCarotollaw.inventionchemistry.chemical_compoundlawFood scienceChemical compositionInhibitory activitiebiologyChemistry (all)General MedicineAnti-Bacterial AgentsDaucus carotaStenotrophomonas maltophiliaSeedsMolecular MedicineSesquiterpenes030106 microbiologyBioengineeringPyrogallolGram-Positive BacteriaSesquiterpeneSteam distillation03 medical and health sciencesAnti-Bacterial AgentGram-Negative BacteriaBotanyChemical composition; Daucus carota; Essential oils; Foodborne bacteria; Inhibitory activities; Anti-Bacterial Agents; Daucus carota; Gram-Negative Bacteria; Gram-Positive Bacteria; Monoterpenes; Oils Volatile; Pyrogallol; Seeds; Sesquiterpenes; Bioengineering; Chemistry (all); Biochemistry; Molecular Medicine; Molecular BiologyOils VolatileMolecular BiologySeed010405 organic chemistryGeneral ChemistryFoodborne bacteriabiology.organism_classification0104 chemical sciencesMonocyclic SesquiterpeneschemistryMonoterpenesGas chromatographySettore AGR/16 - Microbiologia AgrariaDaucus carotaChemistry & Biodiversity
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How the knowledge of interactions between meningococcus and the human immune system has been used to prepare effective Neisseria meningitidis vaccines

2015

In the last decades, tremendous advancement in dissecting the mechanisms of pathogenicity ofNeisseria meningitidisat a molecular level has been achieved, exploiting converging approaches of different disciplines, ranging from pathology to microbiology, immunology, and omics sciences (such as genomics and proteomics). Here, we review the molecular biology of the infectious agent and, in particular, its interactions with the immune system, focusing on both the innate and the adaptive responses. Meningococci exploit different mechanisms and complex machineries in order to subvert the immune system and to avoid being killed. Capsular polysaccharide and lipooligosaccharide glycan composition, in…

lcsh:Immunologic diseases. AllergyImmunologyGenomicsMeningococcal VaccinesMeningococcal vaccineReview ArticleBiologyMeningitis MeningococcalNeisseria meningitidisProteomicsmedicine.disease_causeImmune systemAntigenConjugate vaccineImmunityAnimals; Gram-Negative Bacteria; Host-Pathogen Interactions; Humans; Immunity; Meningitis Meningococcal; Meningococcal Vaccines; Neisseria meningitidis; Immune System; Immunology and Allergy; ImmunologyGram-Negative BacteriamedicineAnimalsHumansImmunology; Immunology and AllergyImmunology and AllergyNeisseria meningitidisImmunityGeneral MedicineVirologyImmune SystemHost-Pathogen Interactionslcsh:RC581-607
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